H. Pylori IgA
Microplates are coated with H.pylori immunodominant antigens derived from tissue culture of a virulent strain. In the 1st incubation, the solid phase is treated with diluted samples and anti-HP IgA are captured, if present, by the antigens. After washing out all the other components of the sample, in the 2nd incubation bound anti-HP IgA are detected by the addition of anti hIgA antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-HP IgA antibodies present in the sample. IgA in the sample may therefore be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available. Neutralization of IgG anti-HP, carried out directly in the well, is performed in the assay in order to block interferences due to this class in the determination of IgA.
Product Specifications:
- Product code: GDBDHPA
- Sample: 10 µl
- Incubation: 60/ 60 min at 37°C + 20 min RT.
- Shelf Life: 60 weeks