Anti-HBc total
ANTI-HBc TOTAL ELISA is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immune assay) - based on a competitive principle. The solid phase of the microtiter plate is made of polystyrene wells coated with HBcAg and the liquid phase of human peroxidase conjugated Anti-HBc.When a serum or plasma specimen containing Anti-HBc is added to the HBcAg-coated wells together with the human peroxidase conjugated Anti-HBc and incubated, a competition will take place for the binding to the HBcAg on the wells. (HBcAg)-(Anti-HBc • peroxidase) complex and/or (HBcAg)-(Anti-HBc) complex will form on the wells. After washing of the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. Due to the competitive principle a color develops inversely proportional to the amount of Anti-HBc bound to HBcAg deriving from the specimen. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450 nm with a selected reference wavelength within 620 to 690 nm.
Product Specifications:
- Product code: GDBG4CBE3
- Sample: 50 µl
- Sensitivity: 1.869 PEI Units/ml
- Incubation: 60 min/ 30 min
- Calibration: PEI Anti-HBc Total Reference Material